| Gene | Lhx3 |
| Chromosome | 2 |
| Deletion Start | 26,206,435 |
| Deletion End | 26,201,003 |
| Deletion Size | 5,433 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,205,948 | 26,205,968 | GGGTCATGCTGAACTCATCTC |
| TUP | 26,205,918 | 26,205,944 | TGAGTCGAGTCTTAGTGTCTGCACCGG |
| TUR | 26,205,874 | 26,205,894 | TTTCCCTGTAATCGCGTCTTG |
| TDF | 26,201,108 | 26,201,128 | TCCTGGCTGGATGAAGTAGAC |
| TDP | 26,201,085 | 26,201,107 | CATGCTCAGTTCTGACCGAGGCC |
| TDR | 26,201,039 | 26,201,057 | CCAGAGGCAGCACCAAGAC |
PCR Primers
| Primer | Sequence |
| SU | AACGTGAGCAAAGAGAACGC |
| SD | CAGGAAGGACACAAGCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12324A-B9 | VGB6 | C57BL/6NTac | |
| 12324A-D3 | VGB6 | C57BL/6NTac | |
| 12324A-E10 | VGB6 | C57BL/6NTac | |
| 12324A-E11 | VGB6 | C57BL/6NTac | |
| 12324A-H2 | VGB6 | C57BL/6NTac | |
| 12324B-A10 | VGB6 | C57BL/6NTac | |
| 12324B-B3 | VGB6 | C57BL/6NTac | |
| 12324B-C8 | VGB6 | C57BL/6NTac | |
| 12324B-F11 | VGB6 | C57BL/6NTac | |
| 12324B-H7 | VGB6 | C57BL/6NTac | |
