| Gene | Snai2 |
| Chromosome | 16 |
| Deletion Start | 14,705,977 |
| Deletion End | 14,708,303 |
| Deletion Size | 2,327 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,706,309 | 14,706,326 | CTCGCCTGAGCAGCAATC |
| TUP | 14,706,327 | 14,706,350 | TCCTGAAGCCAAGCACTTGGGAAA |
| TUR | 14,706,371 | 14,706,388 | TGCCTGTGGCGGGTAATG |
| TDF | 14,707,402 | 14,707,424 | GAACAGCAAGTGCTCCTTTCCAG |
| TDP | 14,707,425 | 14,707,451 | CCTCTGCTCCAGGGATTGTAAACAGCA |
| TDR | 14,707,456 | 14,707,476 | CTGCATGTGCTTTCCAAAGTC |
PCR Primers
| Primer | Sequence |
| SU | TCCACTGAAATGCCATGAGC |
| SD | TCCTTTCAATCTAGCTGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12328A-F6 | VGB6 | C57BL/6NTac | |
| 12328A-H6 | VGB6 | C57BL/6NTac | |
| 12328A-G4 | VGB6 | C57BL/6NTac | |
| 12328A-H5 | VGB6 | C57BL/6NTac | |
| 12328A-H1 | VGB6 | C57BL/6NTac | |
