| Gene | Foxl2 |
| Chromosome | 9 |
| Deletion Start | 98,955,664 |
| Deletion End | 98,956,887 |
| Deletion Size | 1,224 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,955,872 | 98,955,891 | TCACTCTGTCCGGCATCTAC |
| TUP | 98,955,896 | 98,955,923 | ACATCATAGCCAAGTTCCCGTTCTACGA |
| TUR | 98,955,927 | 98,955,944 | TGCCAGCCCTTCTTGTTC |
| TDF | 98,956,730 | 98,956,749 | GCTCATACTGGGACCACGAC |
| TDP | 98,956,752 | 98,956,771 | CAAGACGGGCGCGCTGCACT |
| TDR | 98,956,774 | 98,956,793 | GGCACTCAGAGATCCAGACG |
PCR Primers
| Primer | Sequence |
| SU | AGCCCATACGAATCAGAACG |
| SD | CACGTTCTTTCCATTCTCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12330A-B5 | VGB6 | C57BL/6NTac | |
| 12330A-B6 | VGB6 | C57BL/6NTac | |
| 12330A-E4 | VGB6 | C57BL/6NTac | |
| 12330B-B4 | VGB6 | C57BL/6NTac | |
| 12330B-D4 | VGB6 | C57BL/6NTac | |
| 12330B-E1 | VGB6 | C57BL/6NTac | |
| 12330B-E7 | VGB6 | C57BL/6NTac | |
| 12330B-F6 | VGB6 | C57BL/6NTac | |
| 12330B-G5 | VGB6 | C57BL/6NTac | |
