| Gene | Nefh |
| Chromosome | 11 |
| Deletion Start | 4,947,931 |
| Deletion End | 4,939,321 |
| Deletion Size | 8,611 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,947,707 | 4,947,725 | GAGCACCGACTCGCTAGAC |
| TUP | 4,947,682 | 4,947,703 | CTAAGCAACGGCCCAGAGGGCT |
| TUR | 4,947,591 | 4,947,611 | TGCCTCACCTTGTCGATGTAG |
| TDF | 4,939,554 | 4,939,574 | CAGCGGCACCAGAGAAGAAAG |
| TDP | 4,939,525 | 4,939,552 | CACCAAGGAGGAGAAGACCACAGAGTCC |
| TDR | 4,939,461 | 4,939,482 | TGGAAAGGCTCTTGTCATCCTC |
PCR Primers
| Primer | Sequence |
| SU | ACCCATCAGTTCTGCAATCC |
| SD | CTGCTCATTCATTATTGCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 135 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12335A-D3 | VGB6 | C57BL/6NTac | |
| 12335A-F4 | VGB6 | C57BL/6NTac | |
| 12335A-F9 | VGB6 | C57BL/6NTac | |
