| Gene | Csrp3 |
| Chromosome | 7 |
| Deletion Start | 48,839,529 |
| Deletion End | 48,830,630 |
| Deletion Size | 8,900 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,839,170 | 48,839,192 | CAGGCTCAAAGTCAGGACAAGTG |
| TUP | 48,839,141 | 48,839,167 | TCTGGCCCTGTTCTCTGTAACTTAGCA |
| TUR | 48,839,102 | 48,839,123 | GCCAAATCTAGCTCCAGACAAC |
| TDF | 48,831,216 | 48,831,239 | TCCCTTCAGCTCCTTTAATCCTTC |
| TDP | 48,831,191 | 48,831,214 | CCAACTCGTCCCACAAGACTCCCA |
| TDR | 48,831,161 | 48,831,183 | AGAGCCACAGCCACATATTAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTAAGCCCACTTCCGATGAG |
| SD | ATCATCTTCCTTTGCATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12338A-H8 | VGB6 | C57BL/6NTac | |
| 12338A-D7 | VGB6 | C57BL/6NTac | |
| 12338A-D8 | VGB6 | C57BL/6NTac | |
| 12338A-G2 | VGB6 | C57BL/6NTac | |
| 12338A-E4 | VGB6 | C57BL/6NTac | |
| 12338A-G4 | VGB6 | C57BL/6NTac | |
