| Gene | Tnxb |
| Chromosome | 17 |
| Deletion Start | 34,670,538 |
| Deletion End | 34,698,383 |
| Deletion Size | 27,846 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,671,080 | 34,671,099 | CAGCCTCCACGGTGTCTTTG |
| TUP | 34,671,100 | 34,671,120 | ACCTGAGCCGCTGCGCCTGTT |
| TUR | 34,671,167 | 34,671,185 | TGAGGTGGGCGTCTTTGTG |
| TDF | 34,697,881 | 34,697,902 | GGCTGAGCTACGATATGCCAAG |
| TDP | 34,697,904 | 34,697,928 | CCTTGAGAGCCAAGGTGACCTGACC |
| TDR | 34,697,940 | 34,697,961 | GGCTTCTCCACGGTCTTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | ATGCTGAGTGCTATGTGGAG |
| SD | GAGCAGGGTTCTTTGTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12342A-D1 | VGB6 | C57BL/6NTac | |
| 12342A-C4 | VGB6 | C57BL/6NTac | |
| 12342A-C3 | VGB6 | C57BL/6NTac | |
| 12342A-A8 | VGB6 | C57BL/6NTac | |
