| Gene | Umod |
| Chromosome | 7 |
| Deletion Start | 119,478,283 |
| Deletion End | 119,463,148 |
| Deletion Size | 15,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,477,459 | 119,477,478 | TGAAGCTGGGCTCTTCTGTC |
| TUP | 119,477,435 | 119,477,458 | CGCAGGACGGTGTTCTGAATGCCA |
| TUR | 119,477,417 | 119,477,434 | GTGCAGGTGGCGTTGTTG |
| TDF | 119,463,253 | 119,463,271 | GAGCATCTGGCTGCTGTTG |
| TDP | 119,463,224 | 119,463,250 | TCCCTCAGCCACTTTGATCTTCATGGT |
| TDR | 119,463,180 | 119,463,198 | AGTGAGCTGGGAGCCACAC |
PCR Primers
| Primer | Sequence |
| SU | ATGGAGGTTTGCGACATAGG |
| SD | AGCAGAACATGCATTGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12348A-B5 | VGB6 | C57BL/6NTac | |
| 12348A-C4 | VGB6 | C57BL/6NTac | |
