| Gene | Tbx19 |
| Chromosome | 1 |
| Deletion Start | 165,160,559 |
| Deletion End | 165,138,953 |
| Deletion Size | 21,607 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 165,160,252 | 165,160,270 | GCAGCAGGCTCTGAACATC |
| TUP | 165,160,227 | 165,160,251 | TTAAAGACAGTCTCGGCTGGCCGTC |
| TUR | 165,160,173 | 165,160,191 | TGGCCTCCATCTTCCACTG |
| TDF | 165,139,191 | 165,139,209 | GGCATCAGGCTCAGAGGTG |
| TDP | 165,139,166 | 165,139,190 | CACGCCAGCACCTCAGGAACAATTC |
| TDR | 165,139,124 | 165,139,146 | GGAGCAAAGAGGAAGGTGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | ACGTCTACTGTAGGCAGGGC |
| SD | TAGGGCCACACACTCTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12350B-B1 | VGB6 | C57BL/6NTac | |
| 12350B-A5 | VGB6 | C57BL/6NTac | |
