| Gene | Hoxa11 |
| Chromosome | 6 |
| Deletion Start | 52,245,716 |
| Deletion End | 52,243,359 |
| Deletion Size | 2,358 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,245,030 | 52,245,047 | TCTTCCGGCCACACTGAG |
| TUP | 52,245,000 | 52,245,022 | CCGGTGGCTCCGGTGCGTATAAA |
| TUR | 52,244,952 | 52,244,970 | GCTGCGCTAGATTCCCAAC |
| TDF | 52,243,884 | 52,243,903 | GCCTCCGTTCAAAGCAGTTC |
| TDP | 52,243,859 | 52,243,880 | CAGCGCAAACTCGCCTGTTGCT |
| TDR | 52,243,838 | 52,243,858 | GCTGAAAGGAAGGCTCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | AAAGAAAGGAAGCCGAGGAG |
| SD | AATCTCCAGTGACCACACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12354A-A11 | VGB6 | C57BL/6NTac | |
| 12354B-A4 | VGB6 | C57BL/6NTac | |
| 12354C-C9 | VGB6 | C57BL/6NTac | |
| 12354C-D7 | VGB6 | C57BL/6NTac | |
