| Gene | Tnf |
| Chromosome | 17 |
| Deletion Start | 35,201,837 |
| Deletion End | 35,200,130 |
| Deletion Size | 1,708 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,201,646 | 35,201,666 | CAAAGGGATGAGGTGAGTGTC |
| TUP | 35,201,621 | 35,201,645 | TGGGCAACCCTTATTCTCGCTCACA |
| TUR | 35,201,589 | 35,201,610 | CTGTCCTTCTTGCCCTCCTAAC |
| TDF | 35,200,483 | 35,200,503 | CCAACGGCATGGATCTCAAAG |
| TDP | 35,200,457 | 35,200,481 | CAACCAACTAGTGGTGCCAGCCGAT |
| TDR | 35,200,432 | 35,200,454 | CCTGGGAGTAGACAAGGTACAAC |
PCR Primers
| Primer | Sequence |
| SU | CAACTTTCCAAACCCTCTGC |
| SD | CTCCCTTTGCAGAACTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12361A-A7 | VGB6 | C57BL/6NTac | |
| 12361A-F12 | VGB6 | C57BL/6NTac | |
| 12361A-C4 | VGB6 | C57BL/6NTac | |
| 12361A-E10 | VGB6 | C57BL/6NTac | |
| 12361A-D5 | VGB6 | C57BL/6NTac | |
| 12361A-B11 | VGB6 | C57BL/6NTac | |
