| Gene | Myh11 |
| Chromosome | 16 |
| Deletion Start | 14,277,893 |
| Deletion End | 14,246,733 |
| Deletion Size | 31,161 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,277,787 | 14,277,807 | GGTAGCCAAGAAGCTGGTGTG |
| TUP | 14,277,761 | 14,277,784 | TCCCTTCAGAGAAGCAGGGCTTCG |
| TUR | 14,277,730 | 14,277,750 | TCGCCCTTCTCCTCCTTGATG |
| TDF | 14,247,125 | 14,247,147 | GGGTTGTAATGGCTTAGCTTGAG |
| TDP | 14,247,094 | 14,247,123 | AAAGTGACACATCAAACTTGCACTGCGGTA |
| TDR | 14,247,042 | 14,247,062 | TGGGATGCCTCTGGGAGTATG |
PCR Primers
| Primer | Sequence |
| SU | TGGTCTGCATACCTCTTTCC |
| SD | GTCGAATGAATGGATCGATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12365A-F7 | VGB6 | C57BL/6NTac | |
| 12365A-B9 | VGB6 | C57BL/6NTac | |
| 12365A-F6 | VGB6 | C57BL/6NTac | |
| 12365A-D5 | VGB6 | C57BL/6NTac | |
