| Gene | Casr |
| Chromosome | 16 |
| Deletion Start | 36,517,783 |
| Deletion End | 36,494,390 |
| Deletion Size | 23,394 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,517,639 | 36,517,660 | GGAGTAGCAGCCAAAGATCAAG |
| TUP | 36,517,606 | 36,517,630 | TCAAGACCAGAGTCTGTGGAGTGCA |
| TUR | 36,517,580 | 36,517,602 | CCTCAATTCAGCCTTTCCTTACC |
| TDF | 36,495,240 | 36,495,263 | TCATCGTCTGGATCTCCTTCATTC |
| TDP | 36,495,217 | 36,495,239 | CAGCCTATGCCAGCACCTACGGC |
| TDR | 36,495,197 | 36,495,214 | TCCACGGCAGAGACAAAC |
PCR Primers
| Primer | Sequence |
| SU | GTGATCCTAAGCCATGCTCC |
| SD | TGTATCTGGACTGTGCCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12374A-D4 | VGB6 | C57BL/6NTac | |
| 12374A-C11 | VGB6 | C57BL/6NTac | |
| 12374A-C10 | VGB6 | C57BL/6NTac | |
