Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

12386 Fshr Status: ES cell colonies screened / QC positives

Gene	Fshr
Chromosome	17
Deletion Start	89,200,609
Deletion End	89,200,467
Deletion Size	143
Genome Build	38

MGI ID	MGI:95583
NCBI Gene ID	14309
BAC ID	448J2
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	89,200,580	89,200,598	GGTCTCCTTGCTGGCATTC
TUP	89,200,549	89,200,574	CTCGGGATCTGGATGTCATCACTGGC
TUR	89,200,516	89,200,536	GGCAGAGGAAGACCCTGTTAG
TDF	89,200,558	89,200,576	GGCTCGGGATCTGGATGTC
TDP	89,200,528	89,200,555	CACTGGCTGTGTCATTGCTCTAACAGGG
TDR	89,200,495	89,200,516	TCTCGGTCACCTTGCTATCTTG

PCR Primers

Primer	Sequence
SU	TCTTGAAGGATAAGACAGGTGC
SD	TCTAAGTGTAGGTCTCCTAAG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	83 bp	+	+	-
TDF	TDR	82 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
12386A-C11	VGB6	C57BL/6NTac
12386A-F9	VGB6	C57BL/6NTac
12386A-H12	VGB6	C57BL/6NTac
12386A-F11	VGB6	C57BL/6NTac