| Gene | Htr2a |
| Chromosome | 14 |
| Deletion Start | 74,641,936 |
| Deletion End | 74,645,184 |
| Deletion Size | 3,249 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,642,005 | 74,642,025 | TCGAGGCTCTACCCTAATGAC |
| TUP | 74,642,028 | 74,642,054 | CAACTCCAGGGATGCTAACACTTCCGA |
| TUR | 74,642,087 | 74,642,104 | CGCAGGAGAGGTTGGTTC |
| TDF | 74,644,791 | 74,644,811 | GAAACCTTCAACCCTGCACTC |
| TDP | 74,644,812 | 74,644,833 | CCCTGCCCGTGCCATCACAGTG |
| TDR | 74,644,870 | 74,644,890 | GGTGACAGGCAAGGGAACTAC |
PCR Primers
| Primer | Sequence |
| SU | TCTCCAAATGCTCCACACTG |
| SD | CACAGTCCAGTAACGATAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12392A-B4 | VGB6 | C57BL/6NTac | |
| 12392A-B5 | VGB6 | C57BL/6NTac | |
| 12392A-G1 | VGB6 | C57BL/6NTac | |
