| Gene | Six6 |
| Chromosome | 12 |
| Deletion Start | 72,940,059 |
| Deletion End | 72,941,872 |
| Deletion Size | 1,814 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,940,432 | 72,940,450 | GGGATGGCGAACAGAAGAC |
| TUP | 72,940,452 | 72,940,473 | CACTGCTTCAAGGAGCGCACGC |
| TUR | 72,940,497 | 72,940,518 | GGTTGGGATATGGGTCCTGAAG |
| TDF | 72,941,186 | 72,941,204 | GCAACTCAGGCCCAAACTC |
| TDP | 72,941,207 | 72,941,232 | CAAGCATCCTCTAGCCTCAGGCATGA |
| TDR | 72,941,273 | 72,941,293 | GCGGGACAGATAAGGCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | TCTAGTCGCAGCCTTGATTG |
| SD | TGGCTTCTTCACACAGAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12393A-B1 | VGB6 | C57BL/6NTac | |
| 12393B-A2 | VGB6 | C57BL/6NTac | |
| 12393B-A5 | VGB6 | C57BL/6NTac | |
| 12393B-A12 | VGB6 | C57BL/6NTac | |
| 12393B-F4 | VGB6 | C57BL/6NTac | |
| 12393B-H1 | VGB6 | C57BL/6NTac | |
