| Gene | Ptgdr |
| Chromosome | 14 |
| Deletion Start | 44,859,250 |
| Deletion End | 44,852,833 |
| Deletion Size | 6,418 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,858,424 | 44,858,445 | CAGTGCTCTTCACCATGTGTTC |
| TUP | 44,858,396 | 44,858,421 | TGCCTTTAATTGTGAGTTGCTGGGCA |
| TUR | 44,858,343 | 44,858,362 | CCCACAAGTTCCCACAGTTC |
| TDF | 44,853,655 | 44,853,674 | TCTTGTGCCGTCCTGGAAAC |
| TDP | 44,853,629 | 44,853,653 | TGAGAATGCAGAACAGCCCTGAGGG |
| TDR | 44,853,604 | 44,853,623 | GACATCGGGACCCATCTGAC |
PCR Primers
| Primer | Sequence |
| SU | GGCCAGCCTTAGACACTCAG |
| SD | TACAAGCCTACAGTGAGACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12394A-A4 | VGB6 | C57BL/6NTac | |
| 12394A-D11 | VGB6 | C57BL/6NTac | |
| 12394A-E2 | VGB6 | C57BL/6NTac | |
