| Gene | Lphn3 |
| Chromosome | 5 |
| Deletion Start | 81,329,789 |
| Deletion End | 81,329,957 |
| Deletion Size | 169 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,329,833 | 81,329,854 | CCCTCCCAGACATCTCCTTCAG |
| TUP | 81,329,855 | 81,329,873 | CAGCCAGCGGCAGAGCGCT |
| TUR | 81,329,874 | 81,329,892 | TGTCCTCGATGAGCCGTTG |
| TDF | 81,329,832 | 81,329,851 | TCCCTCCCAGACATCTCCTT |
| TDP | 81,329,852 | 81,329,870 | CAGCAGCCAGCGGCAGAGC |
| TDR | 81,329,872 | 81,329,891 | GTCCTCGATGAGCCGTTGAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCCTAGGTTTGTCTGAG |
| SD | CTGTCATCCCAGCACTGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 60 bp | + | + | - |
| TDF | TDR | 60 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12400B-B10 | VGB6 | C57BL/6NTac | |
| 12400B-E10 | VGB6 | C57BL/6NTac | |
| 12400B-F12 | VGB6 | C57BL/6NTac | |
