| Gene | Wisp2 |
| Chromosome | 2 |
| Deletion Start | 163,821,325 |
| Deletion End | 163,832,479 |
| Deletion Size | 11,155 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 163,822,173 | 163,822,193 | TTGAGCCAATGCAGTGTTGAG |
| TUP | 163,822,197 | 163,822,221 | AAACATCTCCCTCCCTGGGACCAAC |
| TUR | 163,822,251 | 163,822,269 | CCTCTCCAGCACCATGTTC |
| TDF | 163,832,027 | 163,832,048 | GTCGGCCTTTGCTAGTTGTTTC |
| TDP | 163,832,050 | 163,832,077 | TGACTGATGTCAACAGGATGCATTGTGC |
| TDR | 163,832,105 | 163,832,125 | TCAGTTTCTTCCTGGGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGGGATGGAGGTCTTTCTTG |
| SD | TGGATGTTTCAGGCAATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12404A-G4 | VGB6 | C57BL/6NTac | |
| 12404A-D2 | VGB6 | C57BL/6NTac | |
| 12404A-A3 | VGB6 | C57BL/6NTac | |
