| Gene | Cog7 |
| Chromosome | 7 |
| Deletion Start | 121,981,582 |
| Deletion End | 121,955,935 |
| Deletion Size | 25,648 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,981,339 | 121,981,357 | GGGCCAAGCCTCTCCTTTG |
| TUP | 121,981,308 | 121,981,333 | AAAGCGAGCCCTGCAGTAGTCTGAGT |
| TUR | 121,981,273 | 121,981,292 | GCATCGTCGGACGTGAACAC |
| TDF | 121,956,328 | 121,956,348 | CATGGGCCAGCGATAGAAGTG |
| TDP | 121,956,299 | 121,956,325 | TTTCCATTCAGAATTGAGCTTCGGCCA |
| TDR | 121,956,278 | 121,956,298 | GGACAGGGACAGAGTGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTCTCAGACGTCAATCTTC |
| SD | ACTGAAACCAGTTGCCAGCT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12405B-E11 | VGB6 | C57BL/6NTac | |
| 12405B-G8 | VGB6 | C57BL/6NTac | |
| 12405B-G7 | VGB6 | C57BL/6NTac | |
| 12405B-H5 | VGB6 | C57BL/6NTac | |
| 12405B-G2 | VGB6 | C57BL/6NTac | |
