| Gene | Bmp2 |
| Chromosome | 2 |
| Deletion Start | 133,554,429 |
| Deletion End | 133,554,764 |
| Deletion Size | 336 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,554,532 | 133,554,550 | CATCCAGCCGACCCTTGTC |
| TUP | 133,554,552 | 133,554,573 | CGGCCTTCGGAAGACGTCCTCA |
| TUR | 133,554,582 | 133,554,603 | ACATGCTGAGCAGCCTCAACTC |
| TDF | 133,554,664 | 133,554,684 | ATCTGTACCGCAGGCACTCAG |
| TDP | 133,554,692 | 133,554,710 | AGGAGCGCCCGCCCCAGAC |
| TDR | 133,554,759 | 133,554,777 | CCCGCTCACCTTCGTGATG |
PCR Primers
| Primer | Sequence |
| SU | TTTGTCAGGGATGCAGTGTC |
| SD | GCATGCTGTGCTGACTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12406A-C11 | VGB6 | C57BL/6NTac | |
| 12406A-H3 | VGB6 | C57BL/6NTac | |
| 12406A-F11 | VGB6 | C57BL/6NTac | |
