| Gene | Adamts18 |
| Chromosome | 8 |
| Deletion Start | 113,848,473 |
| Deletion End | 113,844,858 |
| Deletion Size | 3,616 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,847,535 | 113,847,556 | TCTCAGTGTCTCCCACTAGCAG |
| TUP | 113,847,504 | 113,847,530 | AGGACAATTGATGTTTGCCCAGGGCTC |
| TUR | 113,847,477 | 113,847,498 | CATGGTGGCCTGTAAGGTCTTG |
| TDF | 113,844,946 | 113,844,963 | GGACGGTGCTTCAGAGAC |
| TDP | 113,844,913 | 113,844,940 | AGCCTGAGGTACCGCAGTGTTTGTATCA |
| TDR | 113,844,865 | 113,844,885 | CACGTAGACACAGCCACAGAG |
PCR Primers
| Primer | Sequence |
| SU | AGAAATCCCACGCCCTTTAC |
| SD | GCTCCCAGAGACCAGATTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12442A-B4 | VGB6 | C57BL/6NTac | |
| 12442A-F3 | VGB6 | C57BL/6NTac | |
