| Gene | Il1b |
| Chromosome | 2 |
| Deletion Start | 129,370,327 |
| Deletion End | 129,364,973 |
| Deletion Size | 5,355 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 129,369,706 | 129,369,726 | GTTGACGGACCCCAAAAGATG |
| TUP | 129,369,681 | 129,369,705 | AAGGTGAGACTCTGAGCTTGGCTCC |
| TUR | 129,369,657 | 129,369,676 | ATGCCACAGTCCCTCCACAG |
| TDF | 129,365,067 | 129,365,086 | GGGAAACAACAGTGGTCAGG |
| TDP | 129,365,032 | 129,365,060 | TTGACTTCACCATGGAATCCGTGTCTTCC |
| TDR | 129,365,008 | 129,365,027 | AGAAACAGTCCAGCCCATAC |
PCR Primers
| Primer | Sequence |
| SU | TTGCTTCCTTCCAGAGCATC |
| SD | AGCAATGTGCTGGTGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12453B-F2 | VGB6 | C57BL/6NTac | |
| 12453B-B1 | VGB6 | C57BL/6NTac | |
| 12453B-A7 | VGB6 | C57BL/6NTac | |
