| Gene | Thbd |
| Chromosome | 2 |
| Deletion Start | 148,407,943 |
| Deletion End | 148,406,211 |
| Deletion Size | 1,733 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,407,406 | 148,407,426 | GGCCTCTGACGGTGAATACTC |
| TUP | 148,407,381 | 148,407,404 | CGATCCTGAGGCTGCCCACATCTC |
| TUR | 148,407,325 | 148,407,343 | CCGGCAGCGTTTGAAAGTC |
| TDF | 148,406,741 | 148,406,758 | CCGGATGAACCGCACAAG |
| TDP | 148,406,708 | 148,406,733 | TGTTCTGCAATGAAACTTCGTGCCCA |
| TDR | 148,406,664 | 148,406,685 | TCAGGGCATTCACAAACAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTTAAACACAGGCTACCCGC |
| SD | TATCTGGGTCATCTCCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12454A-D10 | VGB6 | C57BL/6NTac | |
| 12454A-F9 | VGB6 | C57BL/6NTac | |
| 12454A-D9 | VGB6 | C57BL/6NTac | |
