| Gene | Ghsr |
| Chromosome | 3 |
| Deletion Start | 27,371,800 |
| Deletion End | 27,374,949 |
| Deletion Size | 3,150 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,372,094 | 27,372,112 | GTCCGCCTCTGGCAGTATC |
| TUP | 27,372,115 | 27,372,137 | CCCTGGAACTTCGGCGACCTGCT |
| TUR | 27,372,153 | 27,372,171 | GCAGCTCTCGCTGACAAAC |
| TDF | 27,374,707 | 27,374,727 | GAGATCGCGCAGATCAGTCAG |
| TDP | 27,374,731 | 27,374,754 | TGCAACCTGGTGTCCTTTGTCCTC |
| TDR | 27,374,802 | 27,374,820 | GGCCACCCGGTACTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | ACAGTCGAGGCTGTATTGGG |
| SD | TGTGATGGAGCTTTGTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12455A-G6 | VGB6 | C57BL/6NTac | |
| 12455A-E7 | VGB6 | C57BL/6NTac | |
