| Gene | Foxo1 |
| Chromosome | 3 |
| Deletion Start | 52,268,805 |
| Deletion End | 52,269,416 |
| Deletion Size | 612 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,268,987 | 52,269,006 | CGCTGTCAGCACCGACTTTA |
| TUP | 52,269,007 | 52,269,028 | TGAGCAACCTGAGCCTGCTGGA |
| TUR | 52,269,030 | 52,269,048 | GCGCGAAGTCCTCACTCTC |
| TDF | 52,269,319 | 52,269,337 | CAGCCGAGAAGAGGCTCAC |
| TDP | 52,269,342 | 52,269,366 | TCGCAGATCTACGAGTGGATGGTGA |
| TDR | 52,269,371 | 52,269,393 | CCTTATCCTTGAAGTAGGGCACG |
PCR Primers
| Primer | Sequence |
| SU | GAACTCGGAGGCTCCTTAG |
| SD | GTCTGGAGTGTCAAATGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12456A-B11 | VGB6 | C57BL/6NTac | |
| 12456A-C8 | VGB6 | C57BL/6NTac | |
| 12456A-E2 | VGB6 | C57BL/6NTac | |
| 12456A-H5 | VGB6 | C57BL/6NTac | |
| 12456A-F9 | VGB6 | C57BL/6NTac | |
| 12456A-F3 | VGB6 | C57BL/6NTac | |
