| Gene | P2ry12 |
| Chromosome | 3 |
| Deletion Start | 59,218,249 |
| Deletion End | 59,217,170 |
| Deletion Size | 1,080 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,218,205 | 59,218,226 | CTCAGCCAATACCACCTTCTCC |
| TUP | 59,218,178 | 59,218,198 | ACCAGCACCCTGTGCGTCAGA |
| TUR | 59,218,156 | 59,218,177 | GAACCTGGGTGATCTTGTAGTC |
| TDF | 59,217,428 | 59,217,446 | AACTCGGGCCGTCTTTGAC |
| TDP | 59,217,404 | 59,217,427 | TGCAGTGCTGAGAACACCCTGTTC |
| TDR | 59,217,380 | 59,217,400 | CCATAGGGTGCTCTCCTTCAC |
PCR Primers
| Primer | Sequence |
| SU | GAAACCAGGAATATGGGCAG |
| SD | TCCAGGCCTTATCCATTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12457B-B11 | VGB6 | C57BL/6NTac | |
| 12457B-H6 | VGB6 | C57BL/6NTac | |
| 12457B-F3 | VGB6 | C57BL/6NTac | |
| 12457B-F4 | VGB6 | C57BL/6NTac | |
| 12457B-F2 | VGB6 | C57BL/6NTac | |
| 12457B-H7 | VGB6 | C57BL/6NTac | |
