| Gene | Tmem64 |
| Chromosome | 4 |
| Deletion Start | 15,265,955 |
| Deletion End | 15,283,412 |
| Deletion Size | 17,458 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 15,266,556 | 15,266,578 | GCGTCCTCATTGGCACCTTTATC |
| TUP | 15,266,581 | 15,266,603 | TCATGTGGTCTGCAAGCGGCTAC |
| TUR | 15,266,639 | 15,266,656 | AACGGCGCTCAGCTTGTC |
| TDF | 15,283,283 | 15,283,305 | CCAGTGGCTCTTCCTTCTACAAC |
| TDP | 15,283,309 | 15,283,334 | AGGACCCTCACGTTTTCTGGAGGTGG |
| TDR | 15,283,369 | 15,283,388 | CAGCACGCTAGGCTCTCAAC |
PCR Primers
| Primer | Sequence |
| SU | GACCACCCAGCTATGTCTCC |
| SD | ACAAGAGCCATTGCGAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12462C-E8 | VGB6 | C57BL/6NTac | |
| 12462A-H10 | VGB6 | C57BL/6NTac | |
