| Gene | 2010305A19Rik |
| Chromosome | 4 |
| Deletion Start | 108,328,188 |
| Deletion End | 108,338,650 |
| Deletion Size | 10,463 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,328,207 | 108,328,226 | AGGACGAGGAGCAGGTGAAG |
| TUP | 108,328,228 | 108,328,252 | CCTTCCTGGAGAACATGGAGGTGGA |
| TUR | 108,328,257 | 108,328,276 | TCGCGGTAGCATTGGTAGTG |
| TDF | 108,338,146 | 108,338,164 | GCTGCCTCTTCCTGCTTTC |
| TDP | 108,338,165 | 108,338,191 | TGATGGCCTGTGAGAAACCAGGAAAGA |
| TDR | 108,338,219 | 108,338,238 | TCCATCATGCGCCAGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGTGTCGGCTTTAGAAC |
| SD | TGGGTTACGTAAGGCAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12463A-D4 | VGB6 | C57BL/6NTac | |
| 12463A-H2 | VGB6 | C57BL/6NTac | |
