| Gene | Gatad1 |
| Chromosome | 5 |
| Deletion Start | 3,647,616 |
| Deletion End | 3,641,308 |
| Deletion Size | 6,309 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,647,576 | 3,647,595 | TGCAGCATGTGCAAGACTAC |
| TUP | 3,647,548 | 3,647,573 | CGTCCTCCATGTGGAAGAAGAGTCCA |
| TUR | 3,647,518 | 3,647,536 | CGTGCAGTGATGGCAGAGG |
| TDF | 3,641,543 | 3,641,564 | TCCAAGGAAGATGGAGTACCTG |
| TDP | 3,641,516 | 3,641,541 | AATTTGTCTGCCACGCGCCTTCTGAG |
| TDR | 3,641,487 | 3,641,507 | GGAAATGGTGAGGACCTTGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGCACACTGGTTCACTC |
| SD | AGCAACAGGCACTAAGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12468A-D4 | VGB6 | C57BL/6NTac | |
| 12468A-E2 | VGB6 | C57BL/6NTac | |
| 12468A-E5 | VGB6 | C57BL/6NTac | |
| 12468A-G1 | VGB6 | C57BL/6NTac | |
