| Gene | Spp1 |
| Chromosome | 5 |
| Deletion Start | 104,436,392 |
| Deletion End | 104,440,702 |
| Deletion Size | 4,311 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,436,557 | 104,436,576 | CTGGCAGCTCAGAGGAGAAG |
| TUP | 104,436,579 | 104,436,607 | AAGCACCTCCGGGTTGATCATTTAACTGA |
| TUR | 104,436,609 | 104,436,626 | CGACGCTGGCGAACATTC |
| TDF | 104,440,262 | 104,440,285 | CACATGAAGAGCGGTGAGTCTAAG |
| TDP | 104,440,287 | 104,440,312 | AGTCCCTCGATGTCATCCCTGTTGCC |
| TDR | 104,440,342 | 104,440,360 | GCTGCCCTTTCCGTTGTTG |
PCR Primers
| Primer | Sequence |
| SU | AAACAAATCTTGGTGTGGAGC |
| SD | CCACCAGACTCCTTACATTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12470A-A6 | VGB6 | C57BL/6NTac | |
| 12470A-A11 | VGB6 | C57BL/6NTac | |
| 12470A-H9 | VGB6 | C57BL/6NTac | |
| 12470B-C12 | VGB6 | C57BL/6NTac | |
| 12470B-H2 | VGB6 | C57BL/6NTac | |
