| Gene | Rplp0 |
| Chromosome | 5 |
| Deletion Start | 115,559,838 |
| Deletion End | 115,563,450 |
| Deletion Size | 3,613 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,560,089 | 115,560,109 | GGGCTGAGACTGGAGTGATTC |
| TUP | 115,560,110 | 115,560,136 | ATCTTGTTGCCCATCTGAGACCTGCCA |
| TUR | 115,560,173 | 115,560,194 | CTGGTTCAAAGCCACCTCAAAG |
| TDF | 115,562,938 | 115,562,956 | AAGCCAGCACCTTACCAAC |
| TDP | 115,562,958 | 115,562,986 | AAGCTGTTAAACCAAGCTCCATGTCCTGT |
| TDR | 115,563,028 | 115,563,047 | GACTAGCTCAGGGCAGACTC |
PCR Primers
| Primer | Sequence |
| SU | AGGACAGTCGATGGAACCAG |
| SD | GCAGACCCAGATACCAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12471A-F11 | VGB6 | C57BL/6NTac | |
| 12471B-A3 | VGB6 | C57BL/6NTac | |
| 12471B-D5 | VGB6 | C57BL/6NTac | |
