| Gene | Akr1b8 |
| Chromosome | 6 |
| Deletion Start | 34,354,234 |
| Deletion End | 34,368,194 |
| Deletion Size | 13,961 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,354,975 | 34,354,997 | TGTTCCATCTCCTAACTTTGTCC |
| TUP | 34,355,008 | 34,355,036 | TTCCTTTCGTGATAACAGGACAGTGGTCA |
| TUR | 34,355,064 | 34,355,084 | AACTCCCAGTCGCCAGAAATC |
| TDF | 34,368,028 | 34,368,051 | AAGTTCAGAGACAACCTGGGATAC |
| TDP | 34,368,053 | 34,368,079 | TGGGACCGTCTCTAAGGAAGCAAGCAT |
| TDR | 34,368,101 | 34,368,120 | GCCACAAGCATGGAGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | GCGCCTAAGGCTCATACACC |
| SD | TATCTGCATGCCCTCAAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12474A-C2 | VGB6 | C57BL/6NTac | |
| 12474A-H6 | VGB6 | C57BL/6NTac | |
| 12474B-G8 | VGB6 | C57BL/6NTac | |
| 12474B-D10 | VGB6 | C57BL/6NTac | |
| 12474C-F6 | VGB6 | C57BL/6NTac | |
| 12474B-C9 | VGB6 | C57BL/6NTac | |
