| Gene | Dmbt1 |
| Chromosome | 7 |
| Deletion Start | 131,032,088 |
| Deletion End | 131,061,352 |
| Deletion Size | 29,265 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,032,421 | 131,032,442 | CCAGGCTCAGGAAAGGCTTATG |
| TUP | 131,032,449 | 131,032,478 | AGGGAGAGGCCTTGCTAAATACATAGGTCC |
| TUR | 131,032,506 | 131,032,529 | GCACATGCAGAACACCAATACAAG |
| TDF | 131,060,688 | 131,060,708 | GATGGAGGGTGGAACTGTGAC |
| TDP | 131,060,711 | 131,060,732 | TCCTCCCTCATGGCTCCTGGGA |
| TDR | 131,060,780 | 131,060,801 | GCTGGTGTATCATGTGTGCATA |
PCR Primers
| Primer | Sequence |
| SU | AAATCAAGGCCATGTTCAGG |
| SD | TTCATGTCTCACAGTTAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12475A-A2 | VGB6 | C57BL/6NTac | |
| 12475A-E10 | VGB6 | C57BL/6NTac | |
| 12475A-G10 | VGB6 | C57BL/6NTac | |
| 12475A-C2 | VGB6 | C57BL/6NTac | |
