| Gene | Adam8 |
| Chromosome | 7 |
| Deletion Start | 139,992,468 |
| Deletion End | 139,979,410 |
| Deletion Size | 13,059 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,991,586 | 139,991,605 | CTGGTGTGATGGAGCTGGTG |
| TUP | 139,991,557 | 139,991,581 | AGGCACAAAGCTCTTATCTGGGCCA |
| TUR | 139,991,531 | 139,991,552 | AGTGCCAGCAATGGGATCTGAC |
| TDF | 139,980,196 | 139,980,217 | GCCAGGTTCGGGCTTTATCTTC |
| TDP | 139,980,169 | 139,980,195 | CTAGCGGTGAGGCCATGCAGATGTTTC |
| TDR | 139,980,149 | 139,980,168 | GTCTGCACGCAGCTCAGTAG |
PCR Primers
| Primer | Sequence |
| SU | CCAGTTAGCACTCCCAAAGC |
| SD | ATGACGGCAACTTCTTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12476A-A9 | VGB6 | C57BL/6NTac | |
| 12476A-H10 | VGB6 | C57BL/6NTac | |
| 12476A-E5 | VGB6 | C57BL/6NTac | |
| 12476A-D5 | VGB6 | C57BL/6NTac | |
| 12476A-B4 | VGB6 | C57BL/6NTac | |
