| Gene | Grk1 |
| Chromosome | 8 |
| Deletion Start | 13,405,121 |
| Deletion End | 13,416,349 |
| Deletion Size | 11,229 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,405,787 | 13,405,808 | AAACGGCTGAAGAAGAGGAAGG |
| TUP | 13,405,811 | 13,405,832 | TACCAGGTAGGGAGCCCAGCAA |
| TUR | 13,405,834 | 13,405,856 | TCTAGCCTAAGGGCATCCTACTG |
| TDF | 13,416,052 | 13,416,072 | GACAAAGCGGACACAGAGTTC |
| TDP | 13,416,075 | 13,416,100 | CCAAGAATTCGCCTCTGGCAACTGCT |
| TDR | 13,416,102 | 13,416,119 | TCCTCCTGCCAGGGAATG |
PCR Primers
| Primer | Sequence |
| SU | CCAGAGGGAGACAACTGAGC |
| SD | ACCCTGAGCTGACCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12477B-B2 | VGB6 | C57BL/6NTac | |
| 12477B-B7 | VGB6 | C57BL/6NTac | |
| 12477B-G5 | VGB6 | C57BL/6NTac | |
| 12477B-H3 | VGB6 | C57BL/6NTac | |
| 12477B-H4 | VGB6 | C57BL/6NTac | |
| 12477C-F1 | VGB6 | C57BL/6NTac | |
