| Gene | Paqr9 |
| Chromosome | 9 |
| Deletion Start | 95,559,962 |
| Deletion End | 95,561,099 |
| Deletion Size | 1,138 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,560,122 | 95,560,141 | GCTTCATCCTGTCCGGCTAC |
| TUP | 95,560,152 | 95,560,171 | CGTGCACCGCGCAAGAGTGC |
| TUR | 95,560,195 | 95,560,217 | TCCAGAAGTTGAGCGTCTCATTG |
| TDF | 95,560,822 | 95,560,843 | CAACGTGAGCAAGATCCCTGAG |
| TDP | 95,560,844 | 95,560,865 | CGCATCCAGCCGGGTCTCTTCG |
| TDR | 95,560,877 | 95,560,896 | TGGAAGAGCTGATGGCTGTG |
PCR Primers
| Primer | Sequence |
| SU | GCGCAGACACCCTAACCC |
| SD | GCTAGACCTCAGAAGCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12480A-A4 | VGB6 | C57BL/6NTac | |
| 12480A-E12 | VGB6 | C57BL/6NTac | |
| 12480A-G4 | VGB6 | C57BL/6NTac | |
