| Gene | Prf1 |
| Chromosome | 10 |
| Deletion Start | 61,299,950 |
| Deletion End | 61,303,932 |
| Deletion Size | 3,983 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,300,001 | 61,300,019 | CCTGCTCCCTGCTACACTG |
| TUP | 61,300,020 | 61,300,046 | CCACTCGGTCAGAATGCAAGCAGAAGC |
| TUR | 61,300,048 | 61,300,065 | ACACCTGGCACGAACTTG |
| TDF | 61,303,440 | 61,303,460 | CCAGTGTGAGTGCCAGGATTC |
| TDP | 61,303,462 | 61,303,486 | AAGGTCACCAACCAGGACTGCTGCC |
| TDR | 61,303,516 | 61,303,537 | GTTCTGCCCGGAAATTGCTTAC |
PCR Primers
| Primer | Sequence |
| SU | ATCTCGATTCCTGCTAGGTC |
| SD | AGGGATTTAAAGCTGGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12481A-B6 | VGB6 | C57BL/6NTac | |
| 12481A-C12 | VGB6 | C57BL/6NTac | |
| 12481A-D2 | VGB6 | C57BL/6NTac | |
| 12481A-E11 | VGB6 | C57BL/6NTac | |
| 12481A-G2 | VGB6 | C57BL/6NTac | |
| 12481A-G11 | VGB6 | C57BL/6NTac | |
