| Gene | Shbg |
| Chromosome | 11 |
| Deletion Start | 69,617,859 |
| Deletion End | 69,613,979 |
| Deletion Size | 3,881 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,616,932 | 69,616,952 | CCAAGCACCCATGTTCCAGAG |
| TUP | 69,616,903 | 69,616,928 | CTTCTTTCAGCTGTACTCGGCTGCCC |
| TUR | 69,616,863 | 69,616,884 | GTGGTCAGAAGCAGAGTGAGAG |
| TDF | 69,614,721 | 69,614,743 | GCAAGCGGTGAGATGTATTGAAG |
| TDP | 69,614,686 | 69,614,715 | AACAGCCGTTCTTCCTTTGGATGCTCATGG |
| TDR | 69,614,664 | 69,614,682 | CAAACCCGCCTTTCCTGTG |
PCR Primers
| Primer | Sequence |
| SU | TCAGCATTAGCCTTGACAGG |
| SD | TTCTCCACCTACACGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12485A-A10 | VGB6 | C57BL/6NTac | |
| 12485A-C1 | VGB6 | C57BL/6NTac | |
| 12485A-H9 | VGB6 | C57BL/6NTac | |
| 12485A-D1 | VGB6 | C57BL/6NTac | |
| 12485A-F3 | VGB6 | C57BL/6NTac | |
| 12485A-D7 | VGB6 | C57BL/6NTac | |
