| Gene | Krt10 |
| Chromosome | 11 |
| Deletion Start | 99,389,329 |
| Deletion End | 99,385,590 |
| Deletion Size | 3,740 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,388,904 | 99,388,924 | GTGACCATGCAGAACCTGAAC |
| TUP | 99,388,881 | 99,388,902 | ACCGCCTGGCCTCCTACATGGA |
| TUR | 99,388,843 | 99,388,865 | CCAGCTCGTAGTTTGACTCTTCC |
| TDF | 99,386,488 | 99,386,508 | TCCTCCACCGCTTTCCTTCAG |
| TDP | 99,386,463 | 99,386,487 | CACTGCACATAGCAAAGCTTGCCCA |
| TDR | 99,386,405 | 99,386,429 | GCCCTAATTCCTAAACCCATTCCTG |
PCR Primers
| Primer | Sequence |
| SU | CTTACTGCAATTCGCAAAGC |
| SD | ATACTGGTCCAAGCACACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12487A-F11 | VGB6 | C57BL/6NTac | |
