| Gene | Krt14 |
| Chromosome | 11 |
| Deletion Start | 100,207,452 |
| Deletion End | 100,203,306 |
| Deletion Size | 4,147 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,206,519 | 100,206,538 | GGGAGGAGTGAGCCTGTAAC |
| TUP | 100,206,491 | 100,206,515 | ACCCACCGAGCTAGACACCCAAACC |
| TUR | 100,206,461 | 100,206,481 | GCCTCAGCCCAGACTAACAAG |
| TDF | 100,203,718 | 100,203,741 | GGACTGCTGACCTTAATCTTGTTC |
| TDP | 100,203,687 | 100,203,716 | TCCAATGGGTGTTTGGGTAGATTCCATAGT |
| TDR | 100,203,663 | 100,203,684 | CTCTCCGTGGTGCTGATTTCTG |
PCR Primers
| Primer | Sequence |
| SU | GACGAGAAAGCCCAAAACAC |
| SD | AAAGATCCCATGACCTGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12488A-B3 | VGB6 | C57BL/6NTac | |
| 12488A-C5 | VGB6 | C57BL/6NTac | |
| 12488A-G7 | VGB6 | C57BL/6NTac | |
| 12488A-E5 | VGB6 | C57BL/6NTac | |
| 12488A-E9 | VGB6 | C57BL/6NTac | |
| 12488A-G3 | VGB6 | C57BL/6NTac | |
