| Gene | Krt17 |
| Chromosome | 11 |
| Deletion Start | 100,260,962 |
| Deletion End | 100,256,373 |
| Deletion Size | 4,590 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,260,492 | 100,260,512 | GCATAAACCACCCACCTCCTG |
| TUP | 100,260,464 | 100,260,488 | TCATCAAGGCCTGGAGTCAACTGGG |
| TUR | 100,260,434 | 100,260,458 | CAAACTAGAACCTAGAGACCCTGTG |
| TDF | 100,256,523 | 100,256,543 | TGGCTTTCTTCACCTGCTCAC |
| TDP | 100,256,498 | 100,256,522 | AGCCCGAGTGTCTGCTTCTCTTGCA |
| TDR | 100,256,446 | 100,256,467 | TGCCATCCTGAACTTCTTCCAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGGTGAAATGAAGCAGAGC |
| SD | TTTAGCCTGAACCACCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12489A-A8 | VGB6 | C57BL/6NTac | |
| 12489A-H10 | VGB6 | C57BL/6NTac | |
| 12489A-G9 | VGB6 | C57BL/6NTac | |
| 12489A-H9 | VGB6 | C57BL/6NTac | |
| 12489A-E11 | VGB6 | C57BL/6NTac | |
