| Gene | Gcgr |
| Chromosome | 11 |
| Deletion Start | 120,534,760 |
| Deletion End | 120,538,737 |
| Deletion Size | 3,978 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,535,211 | 120,535,232 | ATGCAGAGCTGCCTCCTAGTTG |
| TUP | 120,535,235 | 120,535,255 | CCTGCTGTCCTCCCTGCTGGA |
| TUR | 120,535,333 | 120,535,352 | TGCCCAGTGTGAGTGATGAC |
| TDF | 120,538,154 | 120,538,176 | AGGAGGTAGGTGAAGCTGGGAAC |
| TDP | 120,538,178 | 120,538,200 | CAATCAGAGCTGGCCCTGAGGGT |
| TDR | 120,538,243 | 120,538,262 | GACATCCAACACATGCAACC |
PCR Primers
| Primer | Sequence |
| SU | GAACTGACCTGGAATGAGGG |
| SD | GGCAGACTTCCTCAAACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 142 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12492A-D4 | VGB6 | C57BL/6NTac | |
| 12492A-D6 | VGB6 | C57BL/6NTac | |
| 12492A-E3 | VGB6 | C57BL/6NTac | |
| 12492A-E4 | VGB6 | C57BL/6NTac | |
| 12492A-G5 | VGB6 | C57BL/6NTac | |
| 12492A-F4 | VGB6 | C57BL/6NTac | |
