| Gene | Hoxa1 |
| Chromosome | 6 |
| Deletion Start | 52,158,218 |
| Deletion End | 52,156,632 |
| Deletion Size | 1,587 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,158,161 | 52,158,179 | CTTGGCAGTGGCGACTCTG |
| TUP | 52,158,138 | 52,158,158 | ACCTGCTCGGCGCGAGCTTAC |
| TUR | 52,158,099 | 52,158,118 | CGCGCAGGATTGGAAAGTTG |
| TDF | 52,156,941 | 52,156,959 | GATTGCCGCGTCCCTACAG |
| TDP | 52,156,909 | 52,156,935 | TGAGACCCAGGTGAAGATCTGGTTCCA |
| TDR | 52,156,871 | 52,156,890 | CCTCCTTCTCACGCTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGCCACTGAAACGGTGATC |
| SD | AACTCACACACATGAAACCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12495A-F4 | VGB6 | C57BL/6NTac | |
| 12495A-G2 | VGB6 | C57BL/6NTac | |
