| Gene | Prok2 |
| Chromosome | 6 |
| Deletion Start | 99,726,317 |
| Deletion End | 99,712,339 |
| Deletion Size | 13,979 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,725,691 | 99,725,715 | CCTTTCACCGTGAAGTTCTCCTTAC |
| TUP | 99,725,649 | 99,725,677 | TATGACTCCTGATACCTCACTCCTAGGGC |
| TUR | 99,725,624 | 99,725,643 | TAGCAACAGCCTGCTCCATC |
| TDF | 99,712,423 | 99,712,441 | GGAGGATGCACCACACCTG |
| TDP | 99,712,394 | 99,712,416 | CCTGCCAGGCTTGGCGTGTTTAA |
| TDR | 99,712,357 | 99,712,377 | TTCCGGGCCAAGCAAATAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGCAAATGATGTGCCTCTG |
| SD | TAAGGGTGTGTCTGTCTGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12498A-A11 | VGB6 | C57BL/6NTac | |
| 12498A-B6 | VGB6 | C57BL/6NTac | |
| 12498A-C1 | VGB6 | C57BL/6NTac | |
| 12498A-D2 | VGB6 | C57BL/6NTac | |
