| Gene | Phox2a |
| Chromosome | 7 |
| Deletion Start | 101,818,501 |
| Deletion End | 101,822,196 |
| Deletion Size | 3,696 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,819,032 | 101,819,053 | TGACCACCAAACCTCTCCATTC |
| TUP | 101,819,060 | 101,819,087 | TCCTCAGAATTGGCTTCCAGCTCAGTCC |
| TUR | 101,819,110 | 101,819,129 | TGGCATCACCCAAGACACAG |
| TDF | 101,822,072 | 101,822,092 | CAGGTCCCTTCTCTGGAGTTC |
| TDP | 101,822,093 | 101,822,115 | TGTCCTCCTTTCACCGGAAGCCC |
| TDR | 101,822,130 | 101,822,149 | GCGGCTAGAAGAGGTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | ATAAGGGACTCTCGGATTGG |
| SD | GGGACTCTTCAGGTTGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12504A-D9 | VGB6 | C57BL/6NTac | |
| 12504A-F1 | VGB6 | C57BL/6NTac | |
| 12504A-B10 | VGB6 | C57BL/6NTac | |
| 12504A-E3 | VGB6 | C57BL/6NTac | |
