| Gene | 2900092E17Rik |
| Chromosome | 7 |
| Deletion Start | 127,016,918 |
| Deletion End | 127,015,257 |
| Deletion Size | 1,662 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,016,218 | 127,016,240 | GACACCAAAGGACTCACTGATTG |
| TUP | 127,016,192 | 127,016,216 | CAGGAGACGGACACCAGGTACGGAT |
| TUR | 127,016,123 | 127,016,141 | TCAGACCAGCCCACTTGTG |
| TDF | 127,016,005 | 127,016,029 | GGTCAGTGCCATACTTTCCTATCAG |
| TDP | 127,015,977 | 127,016,000 | CCCGCAGACCTTCCCAGAAGTTCA |
| TDR | 127,015,926 | 127,015,945 | GGTCAGCTCTGGGCATGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCTGCTGAGTAGACGCGAAC |
| SD | CAGTCTCTCCTCTGAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12505B-D12 | VGB6 | C57BL/6NTac | |
| 12505B-F2 | VGB6 | C57BL/6NTac | |
| 12505B-F10 | VGB6 | C57BL/6NTac | |
| 12505B-F8 | VGB6 | C57BL/6NTac | |
