| Gene | Zfp764 |
| Chromosome | 7 |
| Deletion Start | 127,406,601 |
| Deletion End | 127,404,587 |
| Deletion Size | 2,015 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,405,763 | 127,405,780 | CGCACATGCTCAGATGCG |
| TUP | 127,405,739 | 127,405,761 | TGGTCTCTACCGTCCTGTGTCCA |
| TUR | 127,405,703 | 127,405,722 | GCCCCTTAATTTCCACAGCG |
| TDF | 127,405,551 | 127,405,573 | GAGCCACAACTCTCATTTGCTGG |
| TDP | 127,374,564 | 127,374,588 | TCCGCTAGTGGAATTCAAGCCACGG |
| TDR | 127,405,440 | 127,405,461 | GTGTACAGGTGCTCCACCAATG |
PCR Primers
| Primer | Sequence |
| SU | ATGCAGGAAGTCAAGTAATGC |
| SD | CCAGTAACTCAATCCCGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 134 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12506A-A12 | VGB6 | C57BL/6NTac | |
| 12506A-C1 | VGB6 | C57BL/6NTac | |
| 12506A-F1 | VGB6 | C57BL/6NTac | |
| 12506A-G8 | VGB6 | C57BL/6NTac | |
