| Gene | Dlk1 |
| Chromosome | 12 |
| Deletion Start | 109,453,663 |
| Deletion End | 109,460,431 |
| Deletion Size | 6,769 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,454,568 | 109,454,586 | CTAGGCAGGTGGGCTTGTC |
| TUP | 109,454,587 | 109,454,608 | TGGGCTTTCCCAGAGACCTCGA |
| TUR | 109,454,632 | 109,454,656 | GTCATTCTGTCTAGCCTCCCATTTG |
| TDF | 109,459,682 | 109,459,700 | TGGCTTCTCAGGCAACTTC |
| TDP | 109,459,701 | 109,459,726 | TGTGAGATCGTAGCCGCAACCAACAG |
| TDR | 109,459,734 | 109,459,753 | TCGTTCTCGCATGGGTTAGG |
PCR Primers
| Primer | Sequence |
| SU | AGCGGCAGCTCCTCGGCA |
| SD | TTATGGTTTCACGAGGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12507A-C6 | VGB6 | C57BL/6NTac | |
| 12507A-D4 | VGB6 | C57BL/6NTac | |
| 12507A-E8 | VGB6 | C57BL/6NTac | |
| 12507A-F1 | VGB6 | C57BL/6NTac | |
| 12507A-F8 | VGB6 | C57BL/6NTac | |
