| Gene | Nefl |
| Chromosome | 14 |
| Deletion Start | 68,083,966 |
| Deletion End | 68,087,488 |
| Deletion Size | 3,523 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,084,135 | 68,084,154 | CGTCCAGCTCTGGCTCTTTG |
| TUP | 68,084,156 | 68,084,179 | TGCCCAGCCTGGAGAATCTCGATC |
| TUR | 68,084,196 | 68,084,217 | AGACTTGAGGTCGTTGCTGATG |
| TDF | 68,086,613 | 68,086,634 | CTTGATGTCTGCTCGCTCTTTC |
| TDP | 68,086,636 | 68,086,660 | CAGCCTACTATACCAGCCACGTCCA |
| TDR | 68,086,662 | 68,086,682 | CTCGACCTCTGTCTGCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | AGCTCGCTAGCCTTCTGTC |
| SD | TGAGGTTGCACATGAACATTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12509A-D8 | VGB6 | C57BL/6NTac | |
| 12509A-A5 | VGB6 | C57BL/6NTac | |
| 12509A-B1 | VGB6 | C57BL/6NTac | |
| 12509A-D2 | VGB6 | C57BL/6NTac | |
