| Gene | Lhcgr |
| Chromosome | 17 |
| Deletion Start | 88,791,968 |
| Deletion End | 88,764,987 |
| Deletion Size | 26,982 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,791,924 | 88,791,943 | AGCTGCTGGTGCTGGCAATG |
| TUP | 88,791,899 | 88,791,923 | CTGGTGCTGAAGCAGTCACAGCTGC |
| TUR | 88,791,872 | 88,791,890 | AGCGCGACCCTGACAACTC |
| TDF | 88,765,210 | 88,765,232 | TGAATCGCAGGAGCATCTGTAAC |
| TDP | 88,765,186 | 88,765,209 | ACAGGCATCCGGACCCTCCCAGAT |
| TDR | 88,765,122 | 88,765,143 | CGCATAGCAATCAGCCTGATAC |
PCR Primers
| Primer | Sequence |
| SU | CATAGAACCAAGACAAGTCTC |
| SD | TCACCCACAGGATGTCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12514A-D9 | VGB6 | C57BL/6NTac | |
| 12514A-B7 | VGB6 | C57BL/6NTac | |
